Hotspot

ccsA-ndhD

SSC · 116133-116345 · 213 bp

Back to Oenanthe

Pi 0.0324

MarkerSeek score 58.40

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 213
variable_sites: 19
pi: 0.0324
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CTAATGAAGCATGGGGGTCC	GTTTTTGATTCTGGACCGCG	55.0	50.0	58.0	58.0	592-594 bp	1.0	83.4
ccsA-ndhD_p2	GAAGCATGGGGGTCCTATTG	GTTTTTGATTCTGGACCGCG	55.0	50.0	58.0	58.0	587-589 bp	1.0	83.4
ccsA-ndhD_p3	CTAATGAAGCATGGGGGTCC	TACAGGACTCAATGGAGCCA	55.0	50.0	58.0	58.0	977-979 bp	1.0	82.4
ccsA-ndhD_p4	GAAGCATGGGGGTCCTATTG	TACAGGACTCAATGGAGCCA	55.0	50.0	58.0	58.0	972-974 bp	1.0	82.4
ccsA-ndhD_p5	GAAGCATGGGGGTCCTATTG	ACCGCGAGAGTTCTTTGTTT	55.0	45.0	58.0	58.1	573-575 bp	0.857143	77.3