Hotspot

ndhF-rpl32

SSC · 113092-114266 · 1175 bp

Back to Oenanthe

Pi 0.0142

MarkerSeek score 61.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1175
variable_sites: 49
pi: 0.0142
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CCGTGTGAACCAAAACCCTA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	2380-2478 bp	1.0	85.3
ndhF-rpl32_p2	CCGTGTGAACCAAAACCCTA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	2381-2479 bp	1.0	85.3
ndhF-rpl32_p3	CCGTGTGAACCAAAACCCTA	TAGCGGCTTGTTCGAATTCA	50.0	45.0	58.0	57.9	2358-2456 bp	1.0	84.7
ndhF-rpl32_p4	CCGTGTGAACCAAAACCCTA	TTTCACCATAGCGGCTTGTT	50.0	45.0	58.0	58.1	2366-2464 bp	1.0	84.6
ndhF-rpl32_p5	CCGTGTGAACCAAAACCCTA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	2390-2488 bp	1.0	84.3