Hotspot

ycf4-cemA

LSC · 60723-61612 · 890 bp

Back to Oenanthe

Pi 0.0137

MarkerSeek score 61.60

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 890
variable_sites: 35
pi: 0.0137
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CGTGTACCGATTGAAGTATTTTGA	AGGGGTGAATGCTTTCTTTT	37.5	40.0	57.6	55.4	726-961 bp	1.0	47.0
ycf4-cemA_p2	CGTGTACCGATTGAAGTATTTTGA	AGGGGTGAATGCTTTCTTTTT	37.5	38.1	57.6	56.2	726-961 bp	1.0	47.0
ycf4-cemA_p3	CGTGTACCGATTGAAGTATTTTG	AGGGGTGAATGCTTTCTTTT	39.1	40.0	56.3	55.4	726-961 bp	1.0	47.0
ycf4-cemA_p4	CGTGTACCGATTGAAGTATT	AGGGGTGAATGCTTTCTTTT	40.0	40.0	53.2	55.4	726-961 bp	1.0	47.0
ycf4-cemA_p5	CGTGTACCGATTGAAGTATTTTG	AGGGGTGAATGCTTTCTTTTT	39.1	38.1	56.3	56.2	726-961 bp	1.0	47.0