Hotspot

accD-psaI

LSC · 59162-59663 · 502 bp

Back to Oenanthe

Pi 0.0127

MarkerSeek score 58.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 502
variable_sites: 18
pi: 0.0127
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GCTCCACGCTTTCTTTCCTT	AAAGAAGCCATTGCCATTGC	50.0	45.0	58.8	57.9	576-613 bp	1.0	79.5
accD-psaI_p2	GCTCCACGCTTTCTTTCCTT	TTGCCATTGCCGGAAATACT	50.0	45.0	58.8	58.2	566-603 bp	1.0	79.2
accD-psaI_p3	GCTCCACGCTTTCTTTCCT	AAAGAAGCCATTGCCATTGC	52.6	45.0	58.1	57.9	576-613 bp	1.0	77.1
accD-psaI_p4	TCCACGCTTTCTTTCCTTTGA	AAAGAAGCCATTGCCATTGC	42.9	45.0	58.1	57.9	574-611 bp	1.0	77.0
accD-psaI_p5	GCTCCACGCTTTCTTTCCT	TTGCCATTGCCGGAAATACT	52.6	45.0	58.1	58.2	566-603 bp	1.0	76.7