Hotspot

trnF-GAA-ndhJ

LSC · 49314-49699 · 386 bp

Back to Oenanthe

Pi 0.0097

MarkerSeek score 61.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 386
variable_sites: 11
pi: 0.0097
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	TGGATAGGATGGCCTTTACG	55.0	50.0	58.0	56.8	518-526 bp	1.0	73.3
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	GGATAGGATGGCCTTTACGT	55.0	50.0	58.0	56.8	517-525 bp	1.0	73.2
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACG	50.0	50.0	58.1	56.8	482-490 bp	1.0	73.1
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	GGATAGGATGGCCTTTACGT	50.0	50.0	58.1	56.8	481-489 bp	1.0	73.1
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	TGAAAGTTGGATAGGATGGCC	55.0	47.6	58.0	57.7	525-533 bp	1.0	72.6