Hotspot

trnT-GGU-psbD

LSC · 32308-33542 · 1235 bp

Back to Oenanthe

Pi 0.0118
MarkerSeek score 60.30
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1235
variable_sites
38
pi
0.0118
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 AAGGGCCCTTTTAACTCAGC TGGTAAATTTCCCAAGGGCT 50.0 45.0 58.1 57.0 1348-1392 bp 1.0 74.8
trnT-GGU-psbD_p2 GCCCTTTTAACTCAGCGGTA TGGTAAATTTCCCAAGGGCT 50.0 45.0 57.9 57.0 1344-1388 bp 1.0 74.6
trnT-GGU-psbD_p3 TCCGATAAGGGGCTTTAGCT TGGTAAATTTCCCAAGGGCT 50.0 45.0 58.2 57.0 1286-1330 bp 1.0 74.0
trnT-GGU-psbD_p4 GGCGTAAGTCATCGGTTCAA TGGTAAATTTCCCAAGGGCT 50.0 45.0 58.3 57.0 1307-1351 bp 1.0 73.3
trnT-GGU-psbD_p5 AAGGGCCCTTTTAACTCAGC TTGGTAAATTTCCCAAGGGCT 50.0 42.9 58.1 57.7 1349-1393 bp 1.0 72.8