Hotspot

trnC-GCA-petN

LSC · 28249-28956 · 708 bp

Back to Oenanthe

Pi 0.0112

MarkerSeek score 66.80

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 708
variable_sites: 22
pi: 0.0112
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	739-782 bp	1.0	85.6
trnC-GCA-petN_p2	CGATATGGCCGAGTGGTAAG	TTAAAGCAGCCCAAGCAAGA	55.0	45.0	57.9	58.0	779-822 bp	1.0	85.4
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	733-776 bp	1.0	84.2
trnC-GCA-petN_p4	CGATATGGCCGAGTGGTAAG	CAGCCCAAGCAAGACTTACT	55.0	50.0	57.9	57.8	773-816 bp	1.0	84.0
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.4	58.0	757-800 bp	1.0	83.3