Hotspot

trnK-UUU-rps16

LSC · 4137-4930 · 794 bp

Back to Oenanthe

Pi 0.0159

MarkerSeek score 64.20

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 794
variable_sites: 32
pi: 0.0159
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	TCCCTCGCTTCATCCAAATG	AAAGGCGGAGGTTTTTAAGT	50.0	40.0	58.0	55.5	852-903 bp	1.0	66.2
trnK-UUU-rps16_p2	AAAGCCGAGTACTCTACCGT	AAAGGCGGAGGTTTTTAAGT	50.0	40.0	58.2	55.5	808-859 bp	1.0	65.4
trnK-UUU-rps16_p3	AAGCCGAGTACTCTACCGTT	AAAGGCGGAGGTTTTTAAGT	50.0	40.0	58.2	55.5	807-858 bp	1.0	65.4
trnK-UUU-rps16_p4	TCCCTCGCTTCATCCAAATG	AAAAGGCGGAGGTTTTTAAGT	50.0	38.1	58.0	56.3	853-904 bp	1.0	64.6
trnK-UUU-rps16_p5	CCCTCGCTTCATCCAAATGT	AAAGGCGGAGGTTTTTAAGT	50.0	40.0	58.2	55.5	851-902 bp	1.0	64.6