Hotspot

orf532

IRb · 98151-99752 · 1602 bp

Back to Oedogonium

Pi 0.444

MarkerSeek score 67.70

Primer available yes

Label orf532

Feature Metadata

label_name: orf532
feature_type: gene
region: IRb
length_bp: 1602
variable_sites: 472
pi: 0.444
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.13
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf532_p1	GAATTCTACCACACACGGGG	ACAAGTTTTGCAACGTAGCG	55.0	45.0	58.3	57.9	2594 bp	0.25	47.1
orf532_p2	CGGTTGAATGCGGCATTAAA	ACAAGTTTTGCAACGTAGCG	45.0	45.0	57.7	57.9	2615 bp	0.25	47.0
orf532_p3	GCCGTAAATCCTGAACCGAA	ACAAGTTTTGCAACGTAGCG	50.0	45.0	58.3	57.9	2782 bp	0.25	47.0
orf532_p4	ACACGGGGAATTAAAAGGCA	ACAAGTTTTGCAACGTAGCG	45.0	45.0	57.7	57.9	2582 bp	0.25	46.8
orf532_p5	GAATTCTACCACACACGGGG	CAAGTTTTGCAACGTAGCGA	55.0	45.0	58.3	57.6	2593 bp	0.25	44.9