Hotspot

ccsA

LSC · 1-993 · 993 bp

Back to Oedogonium

Pi 0.17

MarkerSeek score 83.90

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: LSC
length_bp: 993
variable_sites: 275
pi: 0.17
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TGACGATCATAACAAAACGA	TTACATCGCCAACCGCTTTA	35.0	45.0	53.0	57.9	628 bp	0.25	10.0
ccsA_p2	TGACGATCATAACAAAACGA	GGCAAAATTCCGGGCATTTT	35.0	45.0	53.0	58.2	793 bp	0.25	10.0
ccsA_p3	TGACGATCATAACAAAACGA	TACATCGCCAACCGCTTTAT	35.0	45.0	53.0	57.7	627 bp	0.25	10.0
ccsA_p4	ATGACGATCATAACAAAACGA	TTACATCGCCAACCGCTTTA	33.3	45.0	53.7	57.9	629 bp	0.25	10.0
ccsA_p5	TGACGATCATAACAAAACGAT	TTACATCGCCAACCGCTTTA	33.3	45.0	53.7	57.9	628 bp	0.25	10.0