Hotspot

trnL(caa)-psaA

SSC · 114909-115496 · 588 bp

Back to Oedogonium

Pi 0.182
MarkerSeek score 85.20
Primer available yes
Label trnL(caa)-psaA

Feature Metadata

label_name
trnL(caa)-psaA
feature_type
igs
region
SSC
length_bp
588
variable_sites
170
pi
0.182
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.93
markerseek_score
85.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL(caa)-psaA_p1 TACCGTTCCATCATAAGGGC ACTCTTTTTGCTTCACTTTCTGG 50.0 39.1 57.1 57.7 646-704 bp 1.0 58.6
trnL(caa)-psaA_p2 TACCGTTCCATCATAAGGGC CTCTTTTTGCTTCACTTTCTGGA 50.0 39.1 57.1 57.5 645-703 bp 1.0 56.6
trnL(caa)-psaA_p3 TACCGTTCCATCATAAGGGC TCTTTTTGCTTCACTTTCTGGAG 50.0 39.1 57.1 57.5 644-702 bp 1.0 56.6
trnL(caa)-psaA_p4 CTACCGTTCCATCATAAGGGC ACTCTTTTTGCTTCACTTTCTGG 52.4 39.1 58.2 57.7 647-705 bp 1.0 56.1
trnL(caa)-psaA_p5 TACCGTTCCATCATAAGGGC TCTTTTTGCTTCACTTTCTGGA 50.0 36.4 57.1 56.4 644-702 bp 1.0 55.8