Hotspot

orf258-orf532

IRb · 92845-98150 · 5306 bp

Back to Oedogonium

Pi 0.46
MarkerSeek score 61.60
Primer available yes
Label orf258-orf532

Feature Metadata

label_name
orf258-orf532
feature_type
igs
region
IRb
length_bp
5306
variable_sites
1764
pi
0.46
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.12
markerseek_score
61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
orf258-orf532_p1 ACACACAAAGGTCTTTCGCT TCAAAATGGGGTGTCTAAGCA 45.0 42.9 58.0 57.5 709 bp 0.25 37.6
orf258-orf532_p2 CGGGTAATACTCAGCCAACC TCAAAATGGGGTGTCTAAGCA 55.0 42.9 58.1 57.5 919 bp 0.25 37.5
orf258-orf532_p3 CACACACAAAGGTCTTTCGC TCAAAATGGGGTGTCTAAGCA 50.0 42.9 57.9 57.5 710 bp 0.25 37.0
orf258-orf532_p4 ACACACAAAGGTCTTTCGCT TGGGGTGTCTAAGCAAAAAGA 45.0 42.9 58.0 57.4 703 bp 0.25 37.0
orf258-orf532_p5 CGGGTAATACTCAGCCAACC TGGGGTGTCTAAGCAAAAAGA 55.0 42.9 58.1 57.4 913 bp 0.25 36.9