Hotspot

ycf1-ycf4

LSC · 56941-57463 · 523 bp

Back to Oedogonium

Pi 0.213

MarkerSeek score 82.70

Primer available yes

Label ycf1-ycf4

Feature Metadata

label_name: ycf1-ycf4
feature_type: igs
region: LSC
length_bp: 523
variable_sites: 173
pi: 0.213
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ycf4_p1	GTCATTGTTCCAATCGCAGC	GGAATGTTGGAGGTGGGTTT	50.0	50.0	58.1	58.0	1310-1316 bp	0.5	60.7
ycf1-ycf4_p2	GTCATTGTTCCAATCGCAGC	TGTTGGAGGTGGGTTTAACG	50.0	50.0	58.1	58.0	1306-1312 bp	0.5	60.6
ycf1-ycf4_p3	GCAGTCATTGTTCCAATCGC	GGAATGTTGGAGGTGGGTTT	50.0	50.0	58.1	58.0	1313 bp	0.25	49.1
ycf1-ycf4_p4	GCAGTCATTGTTCCAATCGC	TGTTGGAGGTGGGTTTAACG	50.0	50.0	58.1	58.0	1309 bp	0.25	49.0
ycf1-ycf4_p5	CGCAGTCATTGTTCCAATCG	GGAATGTTGGAGGTGGGTTT	50.0	50.0	57.8	58.0	1314 bp	0.25	48.6