Hotspot

trnG(ucc)-trnMe(cau)

LSC · 46245-46390 · 146 bp

Back to Oedogonium

Pi 0.146

MarkerSeek score 82.60

Primer available yes

Label trnG(ucc)-trnMe(cau)

Feature Metadata

label_name: trnG(ucc)-trnMe(cau)
feature_type: igs
region: LSC
length_bp: 146
variable_sites: 34
pi: 0.146
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG(ucc)-trnMe(cau)_p1	CGACATTGGACTTGGAAGGA	GGATGCTCTGACCAACTGAG	50.0	55.0	57.5	58.0	225-252 bp	1.0	88.0
trnG(ucc)-trnMe(cau)_p2	CGACATTGGACTTGGAAGGA	ATGAAACGGATGCTCTGACC	50.0	50.0	57.5	58.0	232-259 bp	1.0	88.0
trnG(ucc)-trnMe(cau)_p3	CGACATTGGACTTGGAAGGA	CTAGTGACATCCCGCGTATG	50.0	55.0	57.5	57.9	249-276 bp	1.0	87.8
trnG(ucc)-trnMe(cau)_p4	CGACATTGGACTTGGAAGGA	ACCAACTGAGCTAAACAGGC	50.0	50.0	57.5	58.1	215-242 bp	1.0	87.4
trnG(ucc)-trnMe(cau)_p5	ACATTGGACTTGGAAGGACC	GGATGCTCTGACCAACTGAG	50.0	55.0	57.4	58.0	223-250 bp	1.0	87.1