Hotspot

orf274-atpI

LSC · 30422-31806 · 1385 bp

Back to Oedogonium

Pi 0.0868

MarkerSeek score 84.20

Primer available yes

Label orf274-atpI

Feature Metadata

label_name: orf274-atpI
feature_type: igs
region: LSC
length_bp: 1385
variable_sites: 192
pi: 0.0868
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf274-atpI_p1	CAGAGTGCTTATCTGGCTGG	TCATTAGTAGGGGCCGCTAA	55.0	50.0	58.0	57.9	2272-2431 bp	0.5	59.9
orf274-atpI_p2	CACGTTAGGTGGAGGTTCAG	TCATTAGTAGGGGCCGCTAA	55.0	50.0	57.9	57.9	2471-2630 bp	0.5	59.7
orf274-atpI_p3	CGTTAGGTGGAGGTTCAGTG	TCATTAGTAGGGGCCGCTAA	55.0	50.0	57.9	57.9	2469-2628 bp	0.5	59.7
orf274-atpI_p4	CAGAGTGCTTATCTGGCTGG	CCCCCAAAACGATCCATGAA	55.0	50.0	58.0	58.2	2016-2175 bp	0.5	59.5
orf274-atpI_p5	CAGAGTGCTTATCTGGCTGG	AATCGCCACGGTAATAGAGC	55.0	50.0	58.0	57.8	2230-2389 bp	0.5	59.4