Hotspot

rpoA

SSC · 179804-181024 · 1221 bp

Back to Oedocladium

Pi 0.167

MarkerSeek score 86.70

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: SSC
length_bp: 1221
variable_sites: 194
pi: 0.167
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 86.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	GCGGAAGAGAAGAGTCCTTT	TCCCTAGCGATTCTTCTTTT	50.0	40.0	57.2	54.1	1331 bp	0.5	22.4
rpoA_p2	GCGGAAGAGAAGAGTCCTTT	TTCCCTAGCGATTCTTCTTT	50.0	40.0	57.2	54.1	1332 bp	0.5	22.4
rpoA_p3	GCGGAAGAGAAGAGTCCTTT	TCCCTAGCGATTCTTCTTTTT	50.0	38.1	57.2	54.9	1331 bp	0.5	21.4
rpoA_p4	GCGGAAGAGAAGAGTCCTTT	TTCCCTAGCGATTCTTCTTTT	50.0	38.1	57.2	54.9	1332 bp	0.5	21.4
rpoA_p5	GCGGAAGAGAAGAGTCCTTT	TTCCCTAGCGATTCTTCTTTTT	50.0	36.4	57.2	55.7	1332 bp	0.5	20.0