Hotspot

orf476

LSC · 73884-75314 · 1431 bp

Back to Oedocladium

Pi 0.231

MarkerSeek score 82.60

Primer available yes

Label orf476

Feature Metadata

label_name: orf476
feature_type: gene
region: LSC
length_bp: 1431
variable_sites: 319
pi: 0.231
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf476_p1	TTTTCTTCTGCGGCAGTCAT	GTACCCAATTTCAGGTGGCA	45.0	50.0	58.1	58.1	2713-2806 bp	1.0	88.9
orf476_p2	TTTTCTTCTGCGGCAGTCAT	TGGATTTGTGCATGGACGAA	45.0	45.0	58.1	58.1	2513-2612 bp	1.0	88.8
orf476_p3	TTTTCTTCTGCGGCAGTCAT	ATGGATTTGTGCATGGACGA	45.0	45.0	58.1	57.9	2514-2613 bp	1.0	88.5
orf476_p4	TTTTCTTCTGCGGCAGTCAT	GGAAAGCAAAGGGGAAGTCA	45.0	50.0	58.1	58.0	2294 bp	0.5	59.1
orf476_p5	TAAAGTCGGGTAGGTCGGAG	GGAAAGCAAAGGGGAAGTCA	55.0	50.0	58.2	58.0	1843 bp	0.5	58.0