Hotspot

ccsA

LSC · 163-1467 · 1305 bp

Back to Oedocladium

Pi 0.159

MarkerSeek score 84.20

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: LSC
length_bp: 1305
variable_sites: 143
pi: 0.159
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.69
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GGCGAATTCAGTACCCCAAT	ACAATAATCGCTTCGCTCTT	50.0	40.0	57.9	55.6	1462 bp	0.5	40.1
ccsA_p2	ATTATTTGACAGGGAGCGGC	ACAATAATCGCTTCGCTCTT	50.0	40.0	58.3	55.6	1479 bp	0.5	37.9
ccsA_p3	ACCGCGAAGAATTGCTAAGG	ACAATAATCGCTTCGCTCTT	50.0	40.0	58.6	55.6	1430 bp	0.5	35.5
ccsA_p4	CGCGAAGAATTGCTAAGGGT	ACAATAATCGCTTCGCTCTT	50.0	40.0	58.6	55.6	1428 bp	0.5	35.5
ccsA_p5	GCGAAGAATTGCTAAGGGTG	ACAATAATCGCTTCGCTCTT	50.0	40.0	57.2	55.6	1427 bp	0.5	33.8