Hotspot

rpl36-trnR(ucg)

SSC · 136459-137437 · 979 bp

Back to Oedocladium

Pi 0.402
MarkerSeek score 75.40
Primer available yes
Label rpl36-trnR(ucg)

Feature Metadata

label_name
rpl36-trnR(ucg)
feature_type
igs
region
SSC
length_bp
979
variable_sites
243
pi
0.402
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.62
markerseek_score
75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl36-trnR(ucg)_p1 TATGCAGCAAAATTGGCAGC AAAACGCACCCAAGAGGAAT 45.0 45.0 58.0 58.0 1790 bp 0.5 59.9
rpl36-trnR(ucg)_p2 TATGCAGCAAAATTGGCAGC AAACGCACCCAAGAGGAATT 45.0 45.0 58.0 58.0 1789 bp 0.5 59.9
rpl36-trnR(ucg)_p3 TATGCAGCAAAATTGGCAGC TATCCCATTCAAAGCTGCCC 45.0 50.0 58.0 57.9 2205 bp 0.5 59.3
rpl36-trnR(ucg)_p4 GCAAAATTGGCAGCAGCTAA AAAACGCACCCAAGAGGAAT 45.0 45.0 57.9 58.0 1784 bp 0.5 59.3
rpl36-trnR(ucg)_p5 GCAAAATTGGCAGCAGCTAA AAACGCACCCAAGAGGAATT 45.0 45.0 57.9 58.0 1783 bp 0.5 59.3