Hotspot

orf622-atpB

SSC · 127669-129567 · 1899 bp

Back to Oedocladium

Pi 0.251

MarkerSeek score 73.70

Primer available yes

Label orf622-atpB

Feature Metadata

label_name: orf622-atpB
feature_type: igs
region: SSC
length_bp: 1899
variable_sites: 296
pi: 0.251
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.62
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf622-atpB_p1	CGCCACCTTACTACCCTAGA	TTATCTCGCGGATTAGCTGC	55.0	50.0	57.9	57.9	2508 bp	0.5	59.0
orf622-atpB_p2	CGCCACCTTACTACCCTAGA	TGCTGTTGATCCATTGGCTT	55.0	45.0	57.9	58.1	2473 bp	0.5	58.9
orf622-atpB_p3	CGCCACCTTACTACCCTAGA	CCTGCTCCAGCTACAACATT	55.0	50.0	57.9	57.9	2553 bp	0.5	58.5
orf622-atpB_p4	CGCCACCTTACTACCCTAGA	TCCTGCTGTTGATCCATTGG	55.0	50.0	57.9	57.9	2476 bp	0.5	58.4
orf622-atpB_p5	ATGTCCAATTTGCAAACGCC	TTATCTCGCGGATTAGCTGC	45.0	50.0	58.2	57.9	2524 bp	0.5	57.8