Hotspot

trnR(ucu)-psbA

LSC · 96895-99670 · 2776 bp

Back to Oedocladium

Pi 0.338
MarkerSeek score 68.50
Primer available yes
Label trnR(ucu)-psbA

Feature Metadata

label_name
trnR(ucu)-psbA
feature_type
igs
region
LSC
length_bp
2776
variable_sites
348
pi
0.338
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.37
markerseek_score
68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR(ucu)-psbA_p1 CGATAAGTCGTGGGTTCGAA AAGCATCCGAACCAACCAAT 50.0 45.0 57.7 58.1 1224-2917 bp 1.0 89.2
trnR(ucu)-psbA_p2 CGATAAGTCGTGGGTTCGAA GAGCCCAAGTGCCTTTTACT 50.0 50.0 57.7 58.1 1163-2856 bp 1.0 89.2
trnR(ucu)-psbA_p3 CGATAAGTCGTGGGTTCGAA TGCGATGAATGCGATGATGA 50.0 45.0 57.7 58.1 1279-2972 bp 1.0 88.8
trnR(ucu)-psbA_p4 CGATAAGTCGTGGGTTCGAA CACAAAAACGAGCCCAAGTG 50.0 50.0 57.7 58.2 1172-2865 bp 1.0 88.6
trnR(ucu)-psbA_p5 CGATAAGTCGTGGGTTCGAA CTGCGATGAATGCGATGATG 50.0 50.0 57.7 57.8 1280-2973 bp 1.0 88.0