Hotspot

rps12-psaJ

LSC · 76949-77562 · 614 bp

Back to Oedocladium

Pi 0.292

MarkerSeek score 80.90

Primer available yes

Label rps12-psaJ

Feature Metadata

label_name: rps12-psaJ
feature_type: igs
region: LSC
length_bp: 614
variable_sites: 145
pi: 0.292
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-psaJ_p1	CCACCGCGAACTAAAACAAC	GTGGCATTTGCTTGGATCAC	50.0	50.0	58.0	58.0	955 bp	0.5	59.8
rps12-psaJ_p2	CCACCGCGAACTAAAACAAC	CTTGTGGGGCTCATAATCCC	50.0	55.0	58.0	58.0	1142 bp	0.5	59.7
rps12-psaJ_p3	TCCACCGCGAACTAAAACAA	GTGGCATTTGCTTGGATCAC	45.0	50.0	58.0	58.0	956 bp	0.5	59.6
rps12-psaJ_p4	TCCACCGCGAACTAAAACAA	CTTGTGGGGCTCATAATCCC	45.0	55.0	58.0	58.0	1143 bp	0.5	59.5
rps12-psaJ_p5	CCACCGCGAACTAAAACAAC	ATCCTGCCCCAGCACTATAT	50.0	50.0	58.0	57.9	1106 bp	0.5	59.4