Hotspot

psbL-psbF

LSC · 72632-73061 · 430 bp

Back to Oedocladium

Pi 0.234
MarkerSeek score 81.70
Primer available yes
Label psbL-psbF

Feature Metadata

label_name
psbL-psbF
feature_type
igs
region
LSC
length_bp
430
variable_sites
96
pi
0.234
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbL-psbF_p1 AGTTTATTAGTATCAAATTGGCTCA ACTGCTATGCAATTTATTCAACGT 28.0 33.3 54.8 57.8 483-505 bp 1.0 51.1
psbL-psbF_p2 GTTTATTAGTATCAAATTGGCTCA ACTGCTATGCAATTTATTCAACGT 29.2 33.3 53.4 57.8 482-504 bp 1.0 51.1
psbL-psbF_p3 AGTTTATTAGTATCAAATTGGCTCAT ACTGCTATGCAATTTATTCAACGT 26.9 33.3 55.3 57.8 483-505 bp 1.0 51.1
psbL-psbF_p4 AGTTTATTAGTATCAAATTGGCTCA ACTGCTATGCAATTTATTCAACG 28.0 34.8 54.8 56.2 483-505 bp 1.0 51.1
psbL-psbF_p5 AGTTTATTAGTATCAAATTGGCTCA CTGCTATGCAATTTATTCAACGT 28.0 34.8 54.8 56.2 482-504 bp 1.0 51.1