Hotspot

orf229-rpoBb

LSC · 52118-53522 · 1405 bp

Back to Oedocladium

Pi 0.191

MarkerSeek score 72.40

Primer available yes

Label orf229-rpoBb

Feature Metadata

label_name: orf229-rpoBb
feature_type: igs
region: LSC
length_bp: 1405
variable_sites: 128
pi: 0.191
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.48
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf229-rpoBb_p1	GCCATAGTTCCGAGGAAAGG	CTTCTTGGGCTTGCTGGTTA	55.0	50.0	58.0	58.1	2046 bp	0.5	58.9
orf229-rpoBb_p2	CTAATCCGTGAACGATGCCA	CTTCTTGGGCTTGCTGGTTA	50.0	50.0	58.1	58.1	2062 bp	0.5	58.8
orf229-rpoBb_p3	TGAACGATGCCATAGTTCCG	CTTCTTGGGCTTGCTGGTTA	50.0	50.0	58.1	58.1	2054 bp	0.5	58.8
orf229-rpoBb_p4	GGCTATGTCACACGAAGGTT	CTTCTTGGGCTTGCTGGTTA	50.0	50.0	57.9	58.1	2536 bp	0.5	58.5
orf229-rpoBb_p5	GCCATAGTTCCGAGGAAAGG	CAAAACAAGGCGGTCAAAGG	55.0	50.0	58.0	58.1	1721 bp	0.5	58.5