Hotspot

atpH-atpF

LSC · 45753-46394 · 642 bp

Back to Oedocladium

Pi 0.293

MarkerSeek score 80.70

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: LSC
length_bp: 642
variable_sites: 156
pi: 0.293
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	TCTGTTGTTGCTGCTGGATT	TCTGAAGCTCGTTGATCTGC	45.0	50.0	58.0	58.0	1076 bp	0.5	59.9
atpH-atpF_p2	GCGGCATAGTCAAGTGGTAA	TCTGAAGCTCGTTGATCTGC	50.0	50.0	58.0	58.0	1391 bp	0.5	59.8
atpH-atpF_p3	TCGAATCCCTCCCTTTCCTT	TCTGAAGCTCGTTGATCTGC	50.0	50.0	58.0	58.0	1440 bp	0.5	59.7
atpH-atpF_p4	TTCGAATCCCTCCCTTTCCT	TCTGAAGCTCGTTGATCTGC	50.0	50.0	58.0	58.0	1441 bp	0.5	59.7
atpH-atpF_p5	GTTCGAATCCCTCCCTTTCC	TCTGAAGCTCGTTGATCTGC	55.0	50.0	58.0	58.0	1442 bp	0.5	59.7