Hotspot

trnK(uuu)-trnI(cau)

LSC · 41645-42215 · 571 bp

Back to Oedocladium

Pi 0.338

MarkerSeek score 82.50

Primer available yes

Label trnK(uuu)-trnI(cau)

Feature Metadata

label_name: trnK(uuu)-trnI(cau)
feature_type: igs
region: LSC
length_bp: 571
variable_sites: 143
pi: 0.338
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.74
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK(uuu)-trnI(cau)_p1	ACCGATAAGTTCTGGGTTCG	TGAGTCGATTGCCTAAACCG	50.0	50.0	57.3	58.0	532-642 bp	1.0	86.6
trnK(uuu)-trnI(cau)_p2	ACCGATAAGTTCTGGGTTCG	TGTAGGAGTTGAACCCACCT	50.0	50.0	57.3	57.9	563-673 bp	1.0	85.8
trnK(uuu)-trnI(cau)_p3	CCGATAAGTTCTGGGTTCGA	TGAGTCGATTGCCTAAACCG	50.0	50.0	57.0	58.0	531-641 bp	1.0	84.3
trnK(uuu)-trnI(cau)_p4	TCAATGGTAGAGTACTCGGCT	TGAGTCGATTGCCTAAACCG	47.6	50.0	58.0	58.0	557-667 bp	1.0	83.8
trnK(uuu)-trnI(cau)_p5	AACCGATAAGTTCTGGGTTCG	TGAGTCGATTGCCTAAACCG	47.6	50.0	58.0	58.0	533-643 bp	1.0	83.7