Hotspot

trnY(gua)-trnK(uuu)

LSC · 40647-41572 · 926 bp

Back to Oedocladium

Pi 0.391

MarkerSeek score 78.80

Primer available yes

Label trnY(gua)-trnK(uuu)

Feature Metadata

label_name: trnY(gua)-trnK(uuu)
feature_type: igs
region: LSC
length_bp: 926
variable_sites: 279
pi: 0.391
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY(gua)-trnK(uuu)_p1	ACGCCGCCTTTTAAAGAGAT	TGAGTCGATTGCCTAAACCG	45.0	50.0	57.9	58.0	2214 bp	0.5	59.1
trnY(gua)-trnK(uuu)_p2	ACGCCGCCTTTTAAAGAGAT	TGTAGGAGTTGAACCCACCT	45.0	50.0	57.9	57.9	2245 bp	0.5	58.2
trnY(gua)-trnK(uuu)_p3	ACGCCGCCTTTTAAAGAGAT	TCTTGTCGGCGAATTCAGTT	45.0	45.0	57.9	57.8	1693 bp	0.5	57.8
trnY(gua)-trnK(uuu)_p4	ACGCCGCCTTTTAAAGAGAT	TTCTTGTCGGCGAATTCAGT	45.0	45.0	57.9	57.8	1694 bp	0.5	57.8
trnY(gua)-trnK(uuu)_p5	ACGCCGCCTTTTAAAGAGAT	CTCGGCCAAAAGTGCTAGAA	45.0	50.0	57.9	58.2	2194 bp	0.5	57.6