Hotspot

chlB-chlL

LSC · 30534-31505 · 972 bp

Back to Oedocladium

Pi 0.36

MarkerSeek score 73.70

Primer available yes

Label chlB-chlL

Feature Metadata

label_name: chlB-chlL
feature_type: igs
region: LSC
length_bp: 972
variable_sites: 183
pi: 0.36
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.52
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlB-chlL_p1	GCTGCTTGGTTTTCTCGTTC	CGGGCCACACATCTTCATAA	50.0	50.0	58.0	58.0	1396-1855 bp	1.0	91.4
chlB-chlL_p2	GCTGCTTGGTTTTCTCGTTC	ATTACATCTTCGGGCCACAC	50.0	50.0	58.0	58.0	1406-1865 bp	1.0	91.4
chlB-chlL_p3	GCTGCTTGGTTTTCTCGTTC	TTTTCACGGATAGAGGCTGC	50.0	50.0	58.0	58.0	2141 bp	0.5	59.5
chlB-chlL_p4	CAAGCTGCTTGGTTTTCTCG	TTTTCACGGATAGAGGCTGC	50.0	50.0	58.0	58.0	2144 bp	0.5	59.5
chlB-chlL_p5	ATGCAGCTAAAGAATCGGCA	TTTTCACGGATAGAGGCTGC	45.0	50.0	58.0	58.0	1498 bp	0.5	59.5