Hotspot

ndhB

IRa · 135802-136725 · 924 bp

Back to Odontostemma

Pi 0.137
MarkerSeek score 81.30
Primer available yes
Label ndhB

Feature Metadata

label_name
ndhB
feature_type
gene
region
IRa
length_bp
924
variable_sites
250
pi
0.137
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhB_p1 GTCTCGCGTGTCTACCATTT ATGGCAGAGGCAAATAGAGC 50.0 50.0 58.0 58.0 1684-2344 bp 1.0 91.8
ndhB_p2 CTCGCGTGTCTACCATTTCA ATGGCAGAGGCAAATAGAGC 50.0 50.0 58.0 58.0 1682-2342 bp 1.0 91.8
ndhB_p3 AGATGGAATGGAACGACGTG ATGGCAGAGGCAAATAGAGC 50.0 50.0 58.0 58.0 1346-2120 bp 1.0 91.7
ndhB_p4 GTCTCGCGTGTCTACCATTT TCCGAATTAGTGGATGCTGC 50.0 50.0 58.0 58.0 1750-2410 bp 1.0 91.6
ndhB_p5 GAGTCGGACATCCAATTGCT ATGGCAGAGGCAAATAGAGC 50.0 50.0 58.0 58.0 1540-2163 bp 0.833333 84.9
ndhB_p1 ATGGCAGAGGCAAATAGAGC GTCTCGCGTGTCTACCATTT 50.0 50.0 58.0 58.0 1684-2344 bp 1.0 91.8
ndhB_p2 ATGGCAGAGGCAAATAGAGC CTCGCGTGTCTACCATTTCA 50.0 50.0 58.0 58.0 1682-2342 bp 1.0 91.8
ndhB_p3 ATGGCAGAGGCAAATAGAGC AGATGGAATGGAACGACGTG 50.0 50.0 58.0 58.0 1346-2120 bp 1.0 91.7
ndhB_p4 TCCGAATTAGTGGATGCTGC GTCTCGCGTGTCTACCATTT 50.0 50.0 58.0 58.0 1750-2410 bp 1.0 91.6
ndhB_p5 ATGGCAGAGGCAAATAGAGC GAGTCGGACATCCAATTGCT 50.0 50.0 58.0 58.0 1540-2163 bp 0.833333 84.9