Hotspot

ndhI-rps15

IRa · 116645-118284 · 1640 bp

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Pi 0.17

MarkerSeek score 74.40

Primer available yes

Label ndhI-rps15

Feature Metadata

label_name: ndhI-rps15
feature_type: igs
region: IRa
length_bp: 1640
variable_sites: 529
pi: 0.17
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.67
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-rps15_p1	TTGACCAATGTACCTTGCCG	AAACGTCAACGACTACTGGG	50.0	50.0	58.5	57.9	1322-1802 bp	0.5	64.7
ndhI-rps15_p2	CGTTCTGAGGCGATCCATTT	AAACGTCAACGACTACTGGG	50.0	50.0	58.3	57.9	1884-1885 bp	0.333333	49.5
ndhI-rps15_p3	AAAAGGATTCGTGCATCCCT	AAACGTCAACGACTACTGGG	45.0	50.0	57.5	57.9	2437-2445 bp	0.333333	48.4
ndhI-rps15_p4	CGTTCTGAGGCGATCCATTT	GAGGCTAAATGGGCTGGAAT	50.0	50.0	58.3	57.6	2133-2134 bp	0.333333	47.7
ndhI-rps15_p5	TTGACCAATGTACCTTGCCG	GAGGCTAAATGGGCTGGAAT	50.0	50.0	58.5	57.6	2050-2051 bp	0.333333	46.7