Hotspot

rpl32-trnL-UAG

SSC · 111739-112250 · 512 bp

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Pi 0.107

MarkerSeek score 78.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 512
variable_sites: 107
pi: 0.107
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	ACCAATGGGATTGAAGAGCC	CTGCATAACCCAGGACCATC	50.0	55.0	57.9	58.0	1930-1940 bp	0.333333	53.4
rpl32-trnL-UAG_p2	ACCAATGGGATTGAAGAGCC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.9	57.9	1283-1293 bp	0.333333	53.4
rpl32-trnL-UAG_p3	ACCAATGGGATTGAAGAGCC	ATCCGCATAAAAGAGCTGCA	50.0	45.0	57.9	58.0	1945-1955 bp	0.333333	53.3
rpl32-trnL-UAG_p4	ACCAATGGGATTGAAGAGCC	TCCGCATAAAAGAGCTGCAT	50.0	45.0	57.9	58.0	1944-1954 bp	0.333333	53.3
rpl32-trnL-UAG_p5	ACCAATGGGATTGAAGAGCC	AAAGAGCTGCATAACCCAGG	50.0	50.0	57.9	57.9	1936-1946 bp	0.333333	52.5