Hotspot

psaJ-rpl33

LSC · 61189-61583 · 395 bp

Back to Odontostemma

Pi 0.0526

MarkerSeek score 79.20

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 395
variable_sites: 42
pi: 0.0526
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTTTCCCAGATGCGTTGACA	CGGACATCTTTCCCCTTAGC	45.0	55.0	58.0	58.0	434-465 bp	1.0	91.0
psaJ-rpl33_p2	TTGGGTCTTTAGCCGGTCTA	CGGACATCTTTCCCCTTAGC	50.0	55.0	58.1	58.0	473-504 bp	1.0	90.3
psaJ-rpl33_p3	CGAACTGAACTAAGAGCGCT	CGGACATCTTTCCCCTTAGC	50.0	55.0	58.0	58.0	1064-1105 bp	1.0	89.1
psaJ-rpl33_p4	GCGTTCTACCGAACTGAACT	CGGACATCTTTCCCCTTAGC	50.0	55.0	57.9	58.0	1073-1114 bp	1.0	88.7
psaJ-rpl33_p5	CTGCGCTACATCCCTTTCAT	CGGACATCTTTCCCCTTAGC	50.0	55.0	58.0	58.0	799-844 bp	0.833333	81.9