Hotspot

trnG-GCC-trnfM-CAU

LSC · 33902-34093 · 192 bp

Back to Odontostemma

Pi 0.0497

MarkerSeek score 79.40

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 192
variable_sites: 19
pi: 0.0497
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.8	58.0	243-277 bp	1.0	88.1
trnG-GCC-trnfM-CAU_p2	TCTTTGCCAAGGAGAAGACG	GTCACGGGTTCAAATCCTGT	50.0	50.0	57.8	58.1	237-271 bp	1.0	87.6
trnG-GCC-trnfM-CAU_p3	TCTTTGCCAAGGAGAAGACG	TCACGGGTTCAAATCCTGTC	50.0	50.0	57.8	57.8	236-270 bp	1.0	87.1
trnG-GCC-trnfM-CAU_p4	TCTTTGCCAAGGAGAAGACG	CACGGGTTCAAATCCTGTCT	50.0	50.0	57.8	57.8	235-269 bp	1.0	87.1
trnG-GCC-trnfM-CAU_p5	TCTTTGCCAAGGAGAAGACG	TGAGGTCACGGGTTCAAATC	50.0	50.0	57.8	57.8	241-275 bp	1.0	87.0