Hotspot

ycf1

SSC · 121814-127258 · 5445 bp

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Pi 0.00738

MarkerSeek score 44.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5445
variable_sites: 40
pi: 0.00738
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCAGTCTAAGTAGGAGACAA	AAGACGAACAACAAGGCCAA	40.0	45.0	52.0	58.2	252 bp	1.0	41.6
ycf1_p2	TTCAGTCTAAGTAGGAGACA	AAGACGAACAACAAGGCCAA	40.0	45.0	52.0	58.2	253 bp	1.0	41.6
ycf1_p3	TTCAGTCTAAGTAGGAGACAA	AAGACGAACAACAAGGCCAA	38.1	45.0	53.0	58.2	253 bp	1.0	41.6
ycf1_p4	TTTCAGTCTAAGTAGGAGACA	AAGACGAACAACAAGGCCAA	38.1	45.0	53.0	58.2	254 bp	1.0	41.6
ycf1_p5	AGTCTAAGTAGGAGACAATATACCCT	AAGACGAACAACAAGGCCAA	38.5	45.0	57.9	58.2	250 bp	1.0	41.6