Hotspot

rpl33

LSC · 66183-66383 · 201 bp

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Pi 0.0149

MarkerSeek score 48.90

Primer available yes

Label rpl33

Feature Metadata

label_name: rpl33
feature_type: gene
region: LSC
length_bp: 201
variable_sites: 3
pi: 0.0149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33_p1	TGGTTCGGATTGAAAAGAAAATGA	TCACACACGTATTCTGTTCCA	33.3	42.9	57.6	57.3	291 bp	1.0	40.8
rpl33_p2	TGGTTCGGATTGAAAAGAAA	TCACACACGTATTCTGTTCCA	35.0	42.9	53.3	57.3	291 bp	1.0	40.8
rpl33_p3	TTGGTTCGGATTGAAAAGAA	TCACACACGTATTCTGTTCCA	35.0	42.9	53.3	57.3	292 bp	1.0	40.8
rpl33_p4	TTTGGTTCGGATTGAAAAGA	TCACACACGTATTCTGTTCCA	35.0	42.9	53.3	57.3	293 bp	1.0	40.8
rpl33_p5	TGGTTCGGATTGAAAAGAAAATG	TCACACACGTATTCTGTTCCA	34.8	42.9	56.3	57.3	291 bp	1.0	40.8