Hotspot

ndhA

SSC · 117656-119908 · 2253 bp

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Pi 0.00489

MarkerSeek score 49.00

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2253
variable_sites: 11
pi: 0.00489
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TCCAATGTACCTTGCAGCTC	TAGAGGCCCCAAAAGGAGAA	50.0	50.0	57.9	57.9	2628-2629 bp	1.0	79.1
ndhA_p2	TCCAATGTACCTTGCAGCTC	TAGTAGCGTTTTCCCCTGGA	50.0	50.0	57.9	58.1	2585-2586 bp	1.0	79.1
ndhA_p3	ACGCGAACACATACTTCACA	TAGAGGCCCCAAAAGGAGAA	45.0	50.0	57.9	57.9	2777-2778 bp	1.0	78.9
ndhA_p4	ACGCGAACACATACTTCACA	TAGTAGCGTTTTCCCCTGGA	45.0	50.0	57.9	58.1	2734-2735 bp	1.0	78.8
ndhA_p5	ACGAAAACGCTCTGATGTGA	TAGAGGCCCCAAAAGGAGAA	45.0	50.0	57.9	57.9	2721-2722 bp	1.0	78.8