Hotspot

matK

LSC · 1651-3210 · 1560 bp

Back to Odontochilus

Pi 0.00449

MarkerSeek score 61.20

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1560
variable_sites: 7
pi: 0.00449
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	TGAGCATTTCAGAATACAAATTCA	GCCGTTCTGACCATATTGCA	29.2	50.0	55.4	58.6	1672-1673 bp	1.0	40.3
matK_p2	TGAGCATTTCAGAATACAAATTCA	TAAAATGTACACATGCGCCG	29.2	45.0	55.4	57.2	1688-1689 bp	1.0	40.3
matK_p3	TGAGCATTTCAGAATACAAATTCATG	GCCGTTCTGACCATATTGCA	30.8	50.0	57.1	58.6	1672-1673 bp	1.0	40.3
matK_p4	TGAGCATTTCAGAATACAAATTCATG	TAAAATGTACACATGCGCCG	30.8	45.0	57.1	57.2	1688-1689 bp	1.0	40.3
matK_p5	TGAGCATTTCAGAATACAAATTCA	CCGTTCTGACCATATTGCAC	29.2	50.0	55.4	56.9	1671-1672 bp	1.0	40.3