Hotspot

accD

LSC · 56209-57663 · 1455 bp

Back to Odontochilus

Pi 0.00756

MarkerSeek score 50.90

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1455
variable_sites: 11
pi: 0.00756
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TCAAATAGGGGGCAGAAAGG	ACTGAACCTAGTGCTATACTTCC	50.0	43.5	57.2	57.1	1520-1565 bp	1.0	43.1
accD_p2	CAAATAGGGGGCAGAAAGGC	ACTGAACCTAGTGCTATACTTCC	55.0	43.5	58.9	57.1	1519-1564 bp	1.0	42.6
accD_p3	AAATAGGGGGCAGAAAGGCT	ACTGAACCTAGTGCTATACTTCC	50.0	43.5	59.0	57.1	1518-1563 bp	1.0	41.8
accD_p4	TCAAATAGGGGGCAGAAAGG	CTGAACCTAGTGCTATACTTCCT	50.0	43.5	57.2	56.9	1519-1564 bp	1.0	41.0
accD_p5	CAAATAGGGGGCAGAAAGGC	CTGAACCTAGTGCTATACTTCCT	55.0	43.5	58.9	56.9	1518-1563 bp	1.0	40.6