Hotspot

ndhI-ndhA

SSC · 117545-117655 · 111 bp

Back to Odontochilus

Pi 0.018

MarkerSeek score 62.90

Primer available yes

Label ndhI-ndhA

Feature Metadata

label_name: ndhI-ndhA
feature_type: igs
region: SSC
length_bp: 111
variable_sites: 2
pi: 0.018
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhA_p1	TCCAATGTACCTTGCAGCTC	AGATGGACTTTACCTAGGATGAGA	50.0	41.7	57.9	58.0	300-302 bp	1.0	47.4
ndhI-ndhA_p2	GTCCAATGTACCTTGCAGCT	AGATGGACTTTACCTAGGATGAGA	50.0	41.7	58.2	58.0	301-303 bp	1.0	46.9
ndhI-ndhA_p3	ACCTTGCAGCTCGTATTGTT	AGATGGACTTTACCTAGGATGAGA	45.0	41.7	57.8	58.0	292-294 bp	1.0	46.8
ndhI-ndhA_p4	TCCAATGTACCTTGCAGCTC	TGGACTTTACCTAGGATGAGA	50.0	42.9	57.9	54.8	297-299 bp	1.0	46.3
ndhI-ndhA_p5	TCCAATGTACCTTGCAGCTC	TGACAACTTCTTCTCAACTTGT	50.0	36.4	57.9	55.8	212-214 bp	1.0	46.3