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psbT-psbN

LSC · 73074-73142 · 69 bp

Back to Odontochilus

Pi 0.029

MarkerSeek score 69.20

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 69
variable_sites: 2
pi: 0.029
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	CGAGAACCACCTAAAGTTCCA	CCCTAGTCGCCATCTCCATA	47.6	55.0	57.6	58.1	221 bp	1.0	69.0
psbT-psbN_p2	CGAGAACCACCTAAAGTTCCA	GGGTACGCCTTATATACCGC	47.6	55.0	57.6	57.7	177 bp	1.0	67.5
psbT-psbN_p3	CGAGAACCACCTAAAGTTCCA	GATCCCTTCGAAGAACACGG	47.6	55.0	57.6	58.4	129 bp	1.0	67.2
psbT-psbN_p4	CGAGAACCACCTAAAGTTCCA	TTCGAAGAACACGGGGACTA	47.6	50.0	57.6	58.4	123 bp	1.0	67.1
psbT-psbN_p5	TCGAGAACCACCTAAAGTTCC	CCCTAGTCGCCATCTCCATA	47.6	55.0	57.3	58.1	222 bp	1.0	66.7