Hotspot

ndhC-trnV-UAC

LSC · 49487-50127 · 641 bp

Back to Odontochilus

Pi 0.00313

MarkerSeek score 60.70

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 641
variable_sites: 2
pi: 0.00313
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCCGGAAATGACAAATGCCA	GGTTCGAATCCGTATAGCCC	45.0	55.0	58.1	57.9	750-751 bp	1.0	79.0
ndhC-trnV-UAC_p2	CTGGTCCTTGCCTAATTGGG	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.2	57.9	778-779 bp	1.0	77.8
ndhC-trnV-UAC_p3	TCCGGAAATGACAAATGCCA	CGGTTCGAATCCGTATAGCC	45.0	55.0	58.1	58.3	751-752 bp	1.0	77.2
ndhC-trnV-UAC_p4	CTGGTCCTTGCCTAATTGGG	CGGTTCGAATCCGTATAGCC	55.0	55.0	58.2	58.3	779-780 bp	1.0	76.0
ndhC-trnV-UAC_p5	TCCGGAAATGACAAATGCCA	AGCCCAATGCAACAAAATGC	45.0	45.0	58.1	58.5	843-844 bp	1.0	75.8