Hotspot

trnT-GGU-psbD

LSC · 30841-31848 · 1008 bp

Back to Odontochilus

Pi 0.00896
MarkerSeek score 59.10
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1008
variable_sites
9
pi
0.00896
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTAACGTCATGGTAAGGCGT GAAACGGTCCCTACGTAACC 50.0 55.0 58.0 58.0 1140-1142 bp 1.0 80.9
trnT-GGU-psbD_p2 GTAACGTCATGGTAAGGCGT GGACCAGCCTACAAAAACGA 50.0 50.0 58.0 58.1 1158-1160 bp 1.0 80.0
trnT-GGU-psbD_p3 GTAACGTCATGGTAAGGCGT CGGTCCCTACGTAACCAATC 50.0 55.0 58.0 57.8 1136-1138 bp 1.0 79.2
trnT-GGU-psbD_p4 GTAACGTCATGGTAAGGCGT ACAAAAACGAAACGGTCCCT 50.0 45.0 58.0 58.2 1148-1150 bp 1.0 78.9
trnT-GGU-psbD_p5 GGCGTAAGTCATCGGTTCAA GAAACGGTCCCTACGTAACC 50.0 55.0 58.3 58.0 1125-1127 bp 1.0 78.6