Hotspot

petN-psbM

LSC · 28101-28697 · 597 bp

Back to Odontochilus

Pi 0.0102

MarkerSeek score 66.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 597
variable_sites: 6
pi: 0.0102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	CGTTTATTGCTACTGCGCTG	45.0	50.0	58.0	57.9	749-759 bp	1.0	81.1
petN-psbM_p2	TCTTGCTTGGGCTGCTTTAA	ACGTCAAAACAGTCAGCCAA	45.0	45.0	58.0	58.3	692-702 bp	1.0	79.9
petN-psbM_p3	TCTTGCTTGGGCTGCTTTAA	ATTGCTACTGCGCTGTTCAT	45.0	45.0	58.0	58.3	744-754 bp	1.0	79.7
petN-psbM_p4	AGTAAGTCTTGCTTGGGCTG	CGTTTATTGCTACTGCGCTG	50.0	50.0	57.8	57.9	755-765 bp	1.0	79.6
petN-psbM_p5	TCTTGCTTGGGCTGCTTTAA	TATTGCTACTGCGCTGTTCA	45.0	45.0	58.0	57.6	745-755 bp	1.0	78.7