Hotspot

trnK-UUU-rps16

LSC · 4297-4914 · 618 bp

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Pi 0.013

MarkerSeek score 54.50

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 618
variable_sites: 8
pi: 0.013
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	TCTTGAGCCGTATGAGGAGA	50.0	50.0	58.2	57.6	991 bp	1.0	76.1
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	TCTTGAGCCGTATGAGGAGA	50.0	50.0	58.2	57.6	990 bp	1.0	76.1
trnK-UUU-rps16_p3	AAAGCCGAGTACTCTACCGT	AGACAACGAATTCCGGAACA	50.0	45.0	58.2	57.5	1297-1313 bp	1.0	75.2
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	AGACAACGAATTCCGGAACA	50.0	45.0	58.2	57.5	1296-1312 bp	1.0	75.2
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	AACGAATTCCGGAACAAGGA	50.0	45.0	58.2	57.4	1292-1308 bp	1.0	75.0