Hotspot

rps16

LSC · 5221-6329 · 1109 bp

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Pi 0.00331
MarkerSeek score 47.60
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1109
variable_sites
11
pi
0.00331
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 GGAGTCCCAACGAAAACCAT AAGCAACGCATTTCTTCGTG 50.0 45.0 58.1 58.0 1554-1565 bp 1.0 80.3
rps16_p2 GGGTCATGTGAGAGCAACAA AAGCAACGCATTTCTTCGTG 50.0 45.0 58.1 58.0 1424-1435 bp 1.0 80.3
rps16_p3 TGATACGGGGTCATGTGAGA AAGCAACGCATTTCTTCGTG 50.0 45.0 57.8 58.0 1431-1442 bp 1.0 79.9
rps16_p4 GGAGTCCCAACGAAAACCAT AGTCACTTGCGATCTTTCCC 50.0 50.0 58.1 57.9 1729-1740 bp 1.0 79.7
rps16_p5 GGGTCATGTGAGAGCAACAA AGTCACTTGCGATCTTTCCC 50.0 50.0 58.1 57.9 1599-1610 bp 1.0 79.7