Hotspot

petD

LSC · 80254-81452 · 1199 bp

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Pi 0.00214
MarkerSeek score 55.10
Primer available yes
Label petD

Feature Metadata

label_name
petD
feature_type
gene
region
LSC
length_bp
1199
variable_sites
7
pi
0.00214
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD_p1 TCTCCGAAGAGAAGATGGAT ACGCGTAGTATTTCACGGTT 45.0 45.0 55.0 57.6 1248 bp 1.0 54.0
petD_p2 TCTCCGAAGAGAAGATGGATT ACGCGTAGTATTTCACGGTT 42.9 45.0 55.9 57.6 1248 bp 1.0 52.6
petD_p3 AGTTGAAGTACATTCTCCGAAGA ACGCGTAGTATTTCACGGTT 39.1 45.0 57.3 57.6 1261 bp 1.0 47.8
petD_p4 CTCCGAAGAGAAGATGGATT ACGCGTAGTATTTCACGGTT 45.0 45.0 54.3 57.6 1247 bp 1.0 47.7
petD_p5 TCTCCGAAGAGAAGATGGAT TACGCGTAGTATTTCACGGT 45.0 45.0 55.0 56.8 1249 bp 1.0 47.2