Hotspot

rpl32-ndhF

SSC · 129181-130429 · 1249 bp

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Pi 0.00956
MarkerSeek score 58.80
Primer available yes
Label rpl32-ndhF

Feature Metadata

label_name
rpl32-ndhF
feature_type
igs
region
SSC
length_bp
1249
variable_sites
36
pi
0.00956
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-ndhF_p1 GTACGTTTCTTTGGAACCGC GGAACAAGCAGAAGTCCCAA 50.0 50.0 57.7 58.0 1335-1361 bp 1.0 81.5
rpl32-ndhF_p2 GTACGTTTCTTTGGAACCGC AGCAGAAGTCCCAACCCTAT 50.0 50.0 57.7 58.0 1329-1355 bp 1.0 81.4
rpl32-ndhF_p3 GTACGTTTCTTTGGAACCGC AAGCAGAAGTCCCAACCCTA 50.0 50.0 57.7 58.3 1330-1356 bp 1.0 79.6
rpl32-ndhF_p4 GTACGTTTCTTTGGAACCGC AACAAGCAGAAGTCCCAACC 50.0 50.0 57.7 58.3 1333-1359 bp 1.0 79.2
rpl32-ndhF_p5 TACGTTTCTTTGGAACCGCC GGAACAAGCAGAAGTCCCAA 50.0 50.0 58.8 58.0 1334-1360 bp 1.0 77.7