Hotspot

rpl2-rpl23

LSC · 89478-89506 · 29 bp

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Pi 0.046
MarkerSeek score 55.10
Primer available yes
Label rpl2-rpl23

Feature Metadata

label_name
rpl2-rpl23
feature_type
igs
region
LSC
length_bp
29
variable_sites
3
pi
0.046
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl2-rpl23_p1 CGGTATAGACGCTTATGGCC ATACTGGAACTCGTGGGGAA 55.0 50.0 58.0 58.1 451-455 bp 1.0 80.8
rpl2-rpl23_p2 ATAGACGCTTATGGCCTCCT ATACTGGAACTCGTGGGGAA 50.0 50.0 58.0 58.1 447-451 bp 1.0 80.7
rpl2-rpl23_p3 GCTTATGGCCTCCTCCTCTA ATACTGGAACTCGTGGGGAA 55.0 50.0 58.0 58.1 441-445 bp 1.0 80.7
rpl2-rpl23_p4 TCTGTCCGTGCTTCAAACAA ATACTGGAACTCGTGGGGAA 45.0 50.0 58.0 58.1 890-894 bp 1.0 80.1
rpl2-rpl23_p5 TTCTGTCCGTGCTTCAAACA ATACTGGAACTCGTGGGGAA 45.0 50.0 58.0 58.1 891-895 bp 1.0 80.1