Hotspot

rps19-rpl2

LSC · 87902-87979 · 78 bp

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Pi 0.0244
MarkerSeek score 62.00
Primer available yes
Label rps19-rpl2

Feature Metadata

label_name
rps19-rpl2
feature_type
igs
region
LSC
length_bp
78
variable_sites
4
pi
0.0244
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19-rpl2_p1 TGCCCGAGACCAAGTTACTA GTTATCCTGCGCTTGGAAGA 50.0 50.0 58.1 58.0 264-269 bp 1.0 81.0
rps19-rpl2_p2 TGCCCGAGACCAAGTTACTA CCTGCGCTTGGAAGAAAAAG 50.0 50.0 58.1 57.9 259-264 bp 1.0 80.8
rps19-rpl2_p3 TGCCCGAGACCAAGTTACTA ATCCTGCGCTTGGAAGAAAA 50.0 45.0 58.1 58.1 261-266 bp 1.0 80.5
rps19-rpl2_p4 TGCCCGAGACCAAGTTACTA CACAACCCCTTGGGGTTATC 50.0 55.0 58.1 58.2 278-283 bp 1.0 79.9
rps19-rpl2_p5 TGCCCGAGACCAAGTTACTA GGCCCCGATTGGTAGAAAAA 50.0 50.0 58.1 58.2 302-307 bp 1.0 79.7