Hotspot

ndhC-trnV-UAC

LSC · 52725-54587 · 1863 bp

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Pi 0.0022
MarkerSeek score 60.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1863
variable_sites
12
pi
0.0022
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CTTCACTAATCGGGGCCAAA CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.9 58.1 1999-2008 bp 1.0 79.2
ndhC-trnV-UAC_p2 ACTAATCGGGGCCAAAACTC CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.9 58.1 1995-2004 bp 1.0 79.2
ndhC-trnV-UAC_p3 CACTAATCGGGGCCAAAACT CCGAGAAAGTCTACGGTTCG 50.0 55.0 58.2 58.1 1996-2005 bp 1.0 78.9
ndhC-trnV-UAC_p4 TCACTAATCGGGGCCAAAAC CCGAGAAAGTCTACGGTTCG 50.0 55.0 58.2 58.1 1997-2006 bp 1.0 78.9
ndhC-trnV-UAC_p5 CTTCACTAATCGGGGCCAAA ACGCATGTTGGGTCTTTGAA 50.0 45.0 57.9 58.3 2062-2071 bp 1.0 77.6